Genomic Engineering & Regulation
Like Duolingo, but for Genomic Engineering & Regulation. Tomo turns the whole topic into a game you play five minutes a day, until it actually sticks.
For the part of you with thirty open tabs that never became anything.
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Key ideas in Genomic Engineering & Regulation
- Cas9 requires a specific Protospacer Adjacent Motif (PAM) immediately downstream of the target to initiate binding
- The effective range of a gRNA is limited to ~20bp; distance from the PAM significantly reduces cleavage efficiency
- Engineered Cas variants (like xCas9) can expand the targetable landscape by recognizing non-canonical PAMs
- Non-Homologous End Joining (NHEJ) is error-prone and typically used to disrupt gene function
- Homology-Directed Repair (HDR) requires an exogenous donor template to facilitate precise edits
- HDR efficiency is significantly lower than NHEJ and is restricted to the S and G2 phases of the cell cycle
- Evaluating targetability based on PAM sequence availability
- Predicting the genetic outcome of different DNA repair pathways
- High-fidelity Cas9 variants reduce non-specific interactions
- gRNA design algorithms prioritize minimal homology
- Nickase pairs increase specificity
- Multiplexing targets several loci simultaneously
- Strategies for minimizing unintended genomic cleavage
- Dead-Cas9 (dCas9) has inactivated catalytic domains (HNH and RuvC) so it binds but does not cut
- dCas9 can be fused to transcriptional repressors (KRAB) or activators (VP64) to modulate expression levels
- The application of multiplexing in polygenic research
You've tried the other tabs
Thirty open tabs. Four facts you actually kept.
You watched. You nodded. By Sunday it was gone.
One answer, then back to scrolling.
Eight weeks. You meant to finish. You didn't.
Tomo gives Genomic Engineering & Regulation the Duolingo treatment: levels, streaks, and quick quizzes that test what you just learned. That game loop is what the tabs above never had, so it's the one you actually finish.
Here's what playing it feels like
A real question from this course. Take your best guess.
If your target mutation is 40 base pairs away from the nearest PAM, what is the most likely result for standard Cas9?
Get it right to open this lesson and 11 more in the app.
Where Genomic Engineering & Regulation takes you
Move beyond the basics of inheritance to master the tools of modern genome editing and the complex regulatory layers that dictate cellular identity.
- 1
Engineering the Code
- CRISPR-Cas9 Precision Editing
- Base and Prime Editing
- 2
The Regulatory Architecture
- Epigenetic Landscapes
- The Functional Non-Coding Genome
2 sections · 4 units · 12 levels. Built to play, not to enroll.
You pick the voice
Genomic Engineering & Regulation is taught in the The Bestie style: your friend who just gets it. Want a different feel? In the app you can spin up the same topic in any of Tomo's teaching styles. Same facts, totally different vibe.
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